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2024年12月28日发(作者：个人博客登django)

专利内容由知识产权出版社提供

专利名称：Enzyme amplification by using free enzyme

to release enzyme from an immobilized

enzyme material

发明人：Roger W. Giese,Markus Ehrat,Douglas J.

Cecchini

申请号：US07/516321

申请日：19900430

公开号：US05190864A

公开日：19930302

摘要：A method for amplifying enzyme activity is disclosed. Enzyme amplification is

achieved by covalently bonding enzyme to a supporting material via a molecular chain

which is a substrate for the enzyme, then introducing a small amount of enzyme in the

free state to this system, causing release of a large amount of bound enzyme. In an

alternative embodiment, complementary enzymatically inactive fragments of an active

enzyme, which fragments can recombine to form active enzyme, are covalently attached

to separate support materials by a molecular chain material which is a substrate for the

active enzyme, and these two fragment-supported conjugates are connected in series.

Upon application of free enzyme or free complementary enzyme to one of these

fragment- support conjugates, followed by application of the resulting product mixture

to the second fragment-support conjugate, a large amount of free enzyme is ultimately

produced. In a second alternative embodiment, two different active enzymes are each

attached to separate supporting materials by different leashes, in which the leash for the

first enzyme only is cleaved in the system by the second enzyme, and the leash for the

second enzyme only is cleaved in the system by the first enzyme. These two materials are

connected in series, and upon application of the second enzyme to the first enzyme-

support conjugate, followed by application of the released first enzyme to the second

enzyme-support conjugate material, ultimately a large amount of released active second

enzyme is produced. Also disclosed are molecular conjugates of enzyme material on

supports, required for the enzyme amplification.

申请人：NORTHEASTERN UNIVERSITY

代理机构：Weingarten, Schurgin, Gagnebin & Hayes

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