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2024年12月29日发(作者：编程项目概述)

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专利名称：TARGETED CLEAVAGE OF RNA USING

EUKARYOTIC RIBONUCLEASE P AND

EXTERNAL GUIDE SEQUENCE

发明人：YUAN, Yan,GUERRIER-TAKADA, Cecilia,

L.,ALTMAN, Sidney

申请号：EP93910848.6

申请日：19930428

公开号：EP0638121B1

公开日：19960717

摘要：It has been discovered that any RNA can be targeted for cleavage by RNAase P

from eukaryotic cells, for example, human HeLa cells, using a suitably designed

oligoribonucleotide ('external guide sequence', or EGS) to form a hybrid with the target

RNA, thereby creating a substrate for cleavage by RNAase P in vitro. There are two

classes of EGS that can target RNA for cleavage by human RNAase P. These are prepared

by transcription from a small DNA fragment that contains a sequence coding for an RNA

that can assume a tRNA-like secondary structure. In the first class, the structure lacks at

least the first thirteen nucleotides from the 5' terminus of the tRNA-like sequence, the

anticodon stem and loop, the variable loop or part of the variable loop. The most

efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was

deleted. In the second class, the EGS has changes in both the equivalent of the T-loop

and the anticodon stem of the tRNA-like segment of the EGS. Methods are also disclosed

to randomly select and to express a suitable EGS in vivo to make a selected RNA a target

for cleavage by the host cell RNAase P, thus preventing expression of the function of the

target RNA. The methods and compositions should be useful to prevent the expression

of disease-causing genes in vivo.

申请人：UNIV YALE

地址：US

国籍：US

代理机构：Bassett, Richard Simon

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