16型重组人乳头瘤病毒病毒样颗粒及其制备方法

305 310 315 320

Phe Tyr Asn Pro Asp Thr Gln Arg Leu Val Trp Ala Cys Val Gly Val

325 330 335

Glu Val Gly Arg Gly Gln Pro Leu Gly Val Gly Ile Ser Gly His Pro

340 345 350

Leu Leu Asn Lys Leu Asp Asp Thr Glu Asn Ala Ser Ala Tyr Ala Ala

355 360 365

Asn Ala Gly Val Asp Asn Arg Glu Cys Ile Ser Met Asp Tyr Lys Gln

370 375 380

Thr Gln Leu Cys Leu Ile Gly Cys Lys Pro Pro Ile Gly Glu His Trp

385 390 395 400

Gly Lys Gly Ser Pro Cys Thr Asn Val Ala Val Asn Pro Gly Asp Cys

405 410 415

Pro Pro Leu Glu Leu Ile Asn Thr Val Ile Gln Asp Gly Asp Met Val

420 425 430

Asp Thr Gly Phe Gly Ala Met Asp Phe Thr Thr Leu Gln Ala Asn Lys

435 440 445

Ser Glu Val Pro Leu Asp Ile Cys Thr Ser Ile Cys Lys Tyr Pro Asp

450 455 460

Tyr Ile Lys Met Val Ser Glu Pro Tyr Gly Asp Ser Leu Phe Phe Tyr

465 470 475 480

Leu Arg Arg Glu Gln Met Phe Val Arg His Leu Phe Asn Arg Ala Gly

485 490 495

Ala Val Gly Glu Asn Val Pro Asp Asp Leu Tyr Ile Lys Gly Ser Gly

500 505 510

Ser Thr Ala Asn Leu Ala Ser Ser Asn Tyr Phe Pro Thr Pro Ser Gly

515 520 525

Ser Met Val Thr Ser Asp Ala Gln Ile Phe Asn Lys Pro Tyr Trp Leu

530 535 540

Gln Arg Ala Gln Gly His Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu

545 550 555 560

Phe Val Thr Val Val Asp Thr Thr Arg Ser Thr Asn Met Ser Leu Cys

565 570 575

Ala Ala Ile Ser Thr Ser Glu Thr Thr Tyr Lys Asn Thr Asn Phe Lys

580 585 590

Glu Tyr Leu Arg His Gly Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln

595 600 605

Leu Cys Lys Ile Thr Leu Thr Ala Asp Val Met Thr Tyr Ile His Ser

610 615 620

Met Asn Ser Thr Ile Leu Glu Asp Trp Asn Phe Gly Leu Gln Pro Pro

625 630 635 640

Pro Gly Gly Thr Leu Glu Asp Thr Tyr Arg Phe Val Thr Ser Gln Ala

645 650 655

Ile Ala Cys Gln Lys His Thr Pro Pro Ala Pro Lys Glu Asp Pro Leu

660 665 670

Lys Lys Tyr Thr Phe Trp Glu Val Asn Leu Lys Glu Lys Phe Ser Ala

675 680 685

Asp Leu Asp Gln Phe Pro Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly

690 695 700

Leu Lys Ala Lys Pro Lys Phe Thr Leu Gly Lys Arg Lys Ala Thr Pro

705 710 715 720

Thr Thr Ser Ser Thr Ser Thr Thr Ala Lys Arg Lys Gln Arg Lys Leu

725 730 735

<210>9

<211>830

<212>PRT

<213>人工序列

<400>9

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro

1 5 1015

Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu

202530

Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu

354045

Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys

505560

Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn

65707580

Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu

859095

Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser

100 105 110

Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu

115 120 125

Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn

130 135 140

Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp

145 150 155 160

Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu

165 170 175

Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr

180 185 190

Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala

195 200 205

Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Glu Val Leu

210 215 220

Phe Gln Gly Pro Ser Asp Gln Glu Ala Lys Pro Ser Thr Glu Asp Leu

225 230 235 240

Gly Asp Lys Lys Glu Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln

245 250 255

Asp Ser Ser Glu Ile His Phe Lys Val Lys Met Thr Thr His Leu Lys

260 265 270

Lys Leu Lys Glu Ser Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser

275 280 285

Leu Arg Phe Leu Phe Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro

290 295 300

Lys Glu Leu Gly Met Glu Glu Glu Asp Val Ile Glu Val Tyr Gln Glu

305 310 315 320

Gln Thr Gly Gly Ser Met Ser Leu Trp Leu Pro Ser Glu Ala Thr Val

325 330 335

Tyr Leu Pro Pro Val Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr

340 345 350

Val Ala Arg Thr Asn Ile Tyr Tyr His Ala Gly Thr Ser Arg Leu Leu

355 360 365

Ala Val Gly His Pro Tyr Phe Pro Ile Lys Lys Pro Asn Asn Asn Lys

370 375 380

Ile Leu Val Pro Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile

385 390 395 400

His Leu Pro Asp Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr

405 410 415

Asn Pro Asp Thr Gln Arg Leu Val Trp Ala Cys Val Gly Val Glu Val

420 425 430

Gly Arg Gly Gln Pro Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu

435 440 445

Asn Lys Leu Asp Asp Thr Glu Asn Ala Ser Ala Tyr Ala Ala Asn Ala

450 455 460

Gly Val Asp Asn Arg Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln

465 470 475 480

Leu Cys Leu Ile Gly Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys

485 490 495

Gly Ser Pro Cys Thr Asn Val Ala Val Asn Pro Gly Asp Cys Pro Pro

500 505 510

Leu Glu Leu Ile Asn Thr Val Ile Gln Asp Gly Asp Met Val Asp Thr

515 520 525

Gly Phe Gly Ala Met Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Glu

530 535 540

Val Pro Leu Asp Ile Cys Thr Ser Ile Cys Lys Tyr Pro Asp Tyr Ile

545 550 555 560

Lys Met Val Ser Glu Pro Tyr Gly Asp Ser Leu Phe Phe Tyr Leu Arg

565 570 575

Arg Glu Gln Met Phe Val Arg His Leu Phe Asn Arg Ala Gly Ala Val

580 585 590

Gly Glu Asn Val Pro Asp Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr

595 600 605

Ala Asn Leu Ala Ser Ser Asn Tyr Phe Pro Thr Pro Ser Gly Ser Met

610 615 620

Val Thr Ser Asp Ala Gln Ile Phe Asn Lys Pro Tyr Trp Leu Gln Arg

625 630 635 640

Ala Gln Gly His Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val

645 650 655

Thr Val Val Asp Thr Thr Arg Ser Thr Asn Met Ser Leu Cys Ala Ala

660 665 670

Ile Ser Thr Ser Glu Thr Thr Tyr Lys Asn Thr Asn Phe Lys Glu Tyr

675 680 685

Leu Arg His Gly Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys

690 695 700

Lys Ile Thr Leu Thr Ala Asp Val Met Thr Tyr Ile His Ser Met Asn

705 710 715 720

Ser Thr Ile Leu Glu Asp Trp Asn Phe Gly Leu Gln Pro Pro Pro Gly

725 730 735

Gly Thr Leu Glu Asp Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Ala

740 745 750

Cys Gln Lys His Thr Pro Pro Ala Pro Lys Glu Asp Pro Leu Lys Lys

755 760 765

Tyr Thr Phe Trp Glu Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu

770 775 780

Asp Gln Phe Pro Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu Lys

785 790 795 800

Ala Lys Pro Lys Phe Thr Leu Gly Lys Arg Lys Ala Thr Pro Thr Thr

805 810 815

Ser Ser Thr Ser Thr Thr Ala Lys Arg Lys Gln Arg Lys Leu

820 825 830

<210>10

<211>896

<212>PRT

<213>人工序列

<400>10

Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys

1 5 1015

Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr

202530

Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe

354045

Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala

505560

His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile

65707580

Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp

8590 95

Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu

100 105 110

Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys

115 120 125

Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly

130 135 140

Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro

145 150 155 160

Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys

165 170 175

Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly

180 185 190

Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp

195 200 205

Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala

210 215 220

Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys

225 230 235 240

Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser

245 250 255

Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro

260 265 270

Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp

275 280 285

Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala

290 295 300

Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala

305 310 315 320

Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln

325 330 335

Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala

340 345 350

Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn

355 360 365

Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile

370 375 380

Glu Gly Arg Ile Ser Glu Phe Met Ser Leu Trp Leu Pro Ser Glu Ala

385 390 395 400

Thr Val Tyr Leu Pro Pro Val Pro Val Ser Lys Val Val Ser Thr Asp

405 410 415

Glu Tyr Val Ala Arg Thr Asn Ile Tyr Tyr His Ala Gly Thr Ser Arg

420 425 430

Leu Leu Ala Val Gly His Pro Tyr Phe Pro Ile Lys Lys Pro Asn Asn

435 440 445

Asn Lys Ile Leu Val Pro Lys Val Ser Gly Leu Gln Tyr Arg Val Phe

450 455 460

Arg Ile His Leu Pro Asp Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser

465 470 475 480

Phe Tyr Asn Pro Asp Thr Gln Arg Leu Val Trp Ala Cys Val Gly Val

485 490 495

Glu Val Gly Arg Gly Gln Pro Leu Gly Val Gly Ile Ser Gly His Pro

500 505 510

Leu Leu Asn Lys Leu Asp Asp Thr Glu Asn Ala Ser Ala Tyr Ala Ala

515 520 525

Asn Ala Gly Val Asp Asn Arg Glu Cys Ile Ser Met Asp Tyr Lys Gln

530 535 540

Thr Gln Leu Cys Leu Ile Gly Cys Lys Pro Pro Ile Gly Glu His Trp

545 550 555 560

Gly Lys Gly Ser Pro Cys Thr Asn Val Ala Val Asn Pro Gly Asp Cys

565 570 575

Pro Pro Leu Glu Leu Ile Asn Thr Val Ile Gln Asp Gly Asp Met Val

580 585 590

Asp Thr Gly Phe Gly Ala Met Asp Phe Thr Thr Leu Gln Ala Asn Lys

595 600 605

Ser Glu Val Pro Leu Asp Ile Cys Thr Ser Ile Cys Lys Tyr Pro Asp

610 615 620

Tyr Ile Lys Met Val Ser Glu Pro Tyr Gly Asp Ser Leu Phe Phe Tyr

625 630 635 640

Leu Arg Arg Glu Gln Met Phe Val Arg His Leu Phe Asn Arg Ala Gly

645 650 655

Ala Val Gly Glu Asn Val Pro Asp Asp Leu Tyr Ile Lys Gly Ser Gly

660 665 670

Ser Thr Ala Asn Leu Ala Ser Ser Asn Tyr Phe Pro Thr Pro Ser Gly

675 680 685

Ser Met Val Thr Ser Asp Ala Gln Ile Phe Asn Lys Pro Tyr Trp Leu

690 695 700

Gln Arg Ala Gln Gly His Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu

705 710 715 720

Phe Val Thr Val Val Asp Thr Thr Arg Ser Thr Asn Met Ser Leu Cys

725 730 735

Ala Ala Ile Ser Thr Ser Glu Thr Thr Tyr Lys Asn Thr Asn Phe Lys

740 745 750

Glu Tyr Leu Arg His Gly Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln

755 760 765

Leu Cys Lys Ile Thr Leu Thr Ala Asp Val Met Thr Tyr Ile His Ser

770 775 780

Met Asn Ser Thr Ile Leu Glu Asp Trp Asn Phe Gly Leu Gln Pro Pro

785 790 795 800

Pro Gly Gly Thr Leu Glu Asp Thr Tyr Arg Phe Val Thr Ser Gln Ala

805 810 815

Ile Ala Cys Gln Lys His Thr Pro Pro Ala Pro Lys Glu Asp Pro Leu

820 825 830

Lys Lys Tyr Thr Phe Trp Glu Val Asn Leu Lys Glu Lys Phe Ser Ala

835 840 845

Asp Leu Asp Gln Phe Pro Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly

850 855 860

Leu Lys Ala Lys Pro Lys Phe Thr Leu Gly Lys Arg Lys Ala Thr Pro

865 870 875 880

Thr Thr Ser Ser Thr Ser Thr Thr Ala Lys Arg Lys Gln Arg Lys Leu

885 890 895

<210>11

<211>2097

<212>DNA

<213>人工序列

<400>11

atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60

ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa120

tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat180

ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac240

atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg300

gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt360

gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa420

acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat480

gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa540

aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca600

tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat660

ctggaagttc tgttccaggg gccctctgaa gctaccgttt acctgccgcc ggttccggtt720

tctaaagttg tttctaccga cgaatacgtt gctcgtacca acatctacta ccacgctggt780

acctctcgtc tgctggctgt tggtcacccg tacttcccga tcaaaaaacc gaacaacaac840

aaaatcctgg ttccgaaagt ttctggtctg cagtaccgtg ttttccgtat ccacctgccg900

gacccgaaca aattcggttt cccggacacc tctttctaca acccggacac ccagcgtctg960

gtttgggctt gcgttggtgt tgaagttggt cgtggtcagc cgctgggtgt tggtatctct 1020

ggtcacccgc tgctgaacaa actggacgac accgaaaacg cttctgctta cgctgctaac 1080

gctggtgttg acaaccgtga atgcatctct atggactaca aacagaccca gctgtgcctg 1140

atcggttgca aaccgccgat cggtgaacac tggggtaaag gttctccgtg caccaacgtt 1200

gctgttaacc cgggtgactg cccgccgctg gaactgatca acaccgttat ccaggacggt 1260

gacatggttg acaccggttt cggtgctatg gacttcacca ccctgcaggc taacaaatct 1320

gaagttccgc tggacatctg cacctctatc tgcaaatacc cggactacat caaaatggtt 1380

tctgaaccgt acggtgactc tctgttcttc tacctgcgtc gtgaacagat gttcgttcgt 1440

cacctgttca accgtgctgg tgctgttggt gaaaacgttc cggacgacct gtacatcaaa 1500

ggttctggtt ctaccgctaa cctggcttct tctaactact tcccgacccc gtctggttct 1560

atggttacct ctgacgctca gatcttcaac aaaccgtact ggctgcagcg tgctcagggt 1620

cacaacaacg gtatctgctg gggtaaccag ctgttcgtta ccgttgttga caccacccgt 1680

tctaccaaca tgtctctgtg cgctgctatc tctacctctg aaaccaccta caaaaacacc 1740

aacttcaaag aatacctgcg tcacggtgaa gaatacgacc tgcagttcat cttccagctg 1800

tgcaaaatca ccctgaccgc tgacgttatg acctacatcc actctatgaa ctctaccatc 1860

ctggaagact ggaacttcgg tctgcagccg ccgccgggtg gtaccctgga agacacctac 1920

cgtttcgtta cctctcaggc tatcgcttgc cagaaacaca ccccgccggc tccgaaagaa 1980

gacccgctga aaaaatacac cttctgggaa gttaacctga aagaaaaatt ctctgctgac 2040

ctggaccagt tcccgctggg tcgtaaattc ctgctgcagg ctggtctgaa agcttag2097

<210>12

<211>2472

<212>DNA

<213>人工序列

<400>12

atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60

ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa120

tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat180

ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac240

atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg300

gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt360

gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa420

acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat480

gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa540

aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca600

tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat660

ctggaagttc tgttccaggg gccctctgac caggaagcta aaccgtctac cgaagacctg720

ggtgacaaaa aagaaggtga atacatcaaa ctgaaagtta tcggtcagga ctcttctgaa780

atccacttca aagttaaaat gaccacccac ctgaaaaaac tgaaagaatc ttactgccag840

cgtcagggtg ttccgatgaa ctctctgcgt ttcctgttcg aaggtcagcg tatcgctgac900

aaccacaccc cgaaagaact gggtatggaa gaagaagacg ttatcgaagt ttaccaggaa960

cagaccggtg gatcctctga agctaccgtt tacctgccgc cggttccggt ttctaaagtt 1020

gtttctaccg acgaatacgt tgctcgtacc aacatctact accacgctgg tacctctcgt 1080

ctgctggctg ttggtcaccc gtacttcccg atcaaaaaac cgaacaacaa caaaatcctg 1140

gttccgaaag tttctggtct gcagtaccgt gttttccgta tccacctgcc ggacccgaac 1200

aaattcggtt tcccggacac ctctttctac aacccggaca cccagcgtct ggtttgggct 1260

tgcgttggtg ttgaagttgg tcgtggtcag ccgctgggtg ttggtatctc tggtcacccg 1320

ctgctgaaca aactggacga caccgaaaac gcttctgctt acgctgctaa cgctggtgtt 1380

gacaaccgtg aatgcatctc tatggactac aaacagaccc agctgtgcct gatcggttgc 1440

aaaccgccga tcggtgaaca ctggggtaaa ggttctccgt gcaccaacgt tgctgttaac 1500

ccgggtgact gcccgccgct ggaactgatc aacaccgtta tccaggacgg tgacatggtt 1560

gacaccggtt tcggtgctat ggacttcacc accctgcagg ctaacaaatc tgaagttccg 1620

ctggacatct gcacctctat ctgcaaatac ccggactaca tcaaaatggt ttctgaaccg 1680

tacggtgact ctctgttctt ctacctgcgt cgtgaacaga tgttcgttcg tcacctgttc 1740

aaccgtgctg gtgctgttgg tgaaaacgtt ccggacgacc tgtacatcaa aggttctggt 1800

tctaccgcta acctggcttc ttctaactac ttcccgaccc cgtctggttc tatggttacc 1860

tctgacgctc agatcttcaa caaaccgtac tggctgcagc gtgctcaggg tcacaacaac 1920

ggtatctgct ggggtaacca gctgttcgtt accgttgttg acaccacccg ttctaccaac 1980

atgtctctgt gcgctgctat ctctacctct gaaaccacct acaaaaacac caacttcaaa 2040

gaatacctgc gtcacggtga agaatacgac ctgcagttca tcttccagct gtgcaaaatc 2100

accctgaccg ctgacgttat gacctacatc cactctatga actctaccat cctggaagac 2160

tggaacttcg gtctgcagcc gccgccgggt ggtaccctgg aagacaccta ccgtttcgtt 2220

acctctcagg ctatcgcttg ccagaaacac accccgccgg ctccgaaaga agacccgctg 2280

aaaaaataca ccttctggga agttaacctg aaagaaaaat tctctgctga cctggaccag 2340

ttcccgctgg gtcgtaaatt cctgctgcag gctggtctga aagctaaacc gaaattcacc 2400

ctgggtaaac gtaaagctac cccgaccacc tcttctacct ctaccaccgc taaacgtaaa 2460

cagcgtaaac tg 2472

<210>13

<211>2643

<212>DNA

<213>人工序列

<400>13

atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt 60

ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat120

ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt180

atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc240

accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac300

aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa360

gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg420

aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg480

ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa540

gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt600

aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa660

ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa720

gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt780

ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc840

ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg900

ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc960

actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc 1020

tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa 1080

gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac 1140

aacctcggga tcgagggaag gatttcagaa ttcatgtctc tgtggctgcc gtctgaagct 1200

accgtttacc tgccgccggt tccggtttct aaagttgttt ctaccgacga atacgttgct 1260

cgtaccaaca tctactacca cgctggtacc tctcgtctgc tggctgttgg tcacccgtac 1320

ttcccgatca aaaaaccgaa caacaacaaa atcctggttc cgaaagtttc tggtctgcag 1380

taccgtgttt tccgtatcca cctgccggac ccgaacaaat tcggtttccc ggacacctct 1440

ttctacaacc cggacaccca gcgtctggtt tgggcttgcg ttggtgttga agttggtcgt 1500

ggtcagccgc tgggtgttgg tatctctggt cacccgctgc tgaacaaact ggacgacacc 1560

gaaaacgctt ctgcttacgc tgctaacgct ggtgttgaca accgtgaatg catctctatg 1620

gactacaaac agacccagct gtgcctgatc ggttgcaaac cgccgatcgg tgaacactgg 1680

ggtaaaggtt ctccgtgcac caacgttgct gttaacccgg gtgactgccc gccgctggaa 1740

ctgatcaaca ccgttatcca ggacggtgac atggttgaca ccggtttcgg tgctatggac 1800

ttcaccaccc tgcaggctaa caaatctgaa gttccgctgg acatctgcac ctctatctgc 1860

aaatacccgg actacatcaa aatggtttct gaaccgtacg gtgactctct gttcttctac 1920

ctgcgtcgtg aacagatgtt cgttcgtcac ctgttcaacc gtgctggtgc tgttggtgaa 1980

aacgttccgg acgacctgta catcaaaggt tctggttcta ccgctaacct ggcttcttct 2040

aactacttcc cgaccccgtc tggttctatg gttacctctg acgctcagat cttcaacaaa 2100

ccgtactggc tgcagcgtgc tcagggtcac aacaacggta tctgctgggg taaccagctg 2160

ttcgttaccg ttgttgacac cacccgttct accaacatgt ctctgtgcgc tgctatctct 2220

acctctgaaa ccacctacaa aaacaccaac ttcaaagaat acctgcgtca cggtgaagaa 2280

tacgacctgc agttcatctt ccagctgtgc aaaatcaccc tgaccgctga cgttatgacc 2340

tacatccact ctatgaactc taccatcctg gaagactgga acttcggtct gcagccgccg 2400

ccgggtggta ccctggaaga cacctaccgt ttcgttacct ctcaggctat cgcttgccag 2460

aaacacaccc cgccggctcc gaaagaagac ccgctgaaaa aatacacctt ctgggaagtt 2520

aacctgaaag aaaaattctc tgctgacctg gaccagttcc cgctgggtcg taaattcctg 2580

ctgcaggctg gtctgaaagc taaaccgaaa ttcaccctgg gtaaacgtaa agctaccccg 2640

acc 2643