工业化生物发酵技术——清华大学国际技术转移中心

Biotechnology Catalogue

Version 201306

Note: Most of the following technologies are for A.P.I.s, and they have been successfully

commercialized in the market.

工业化生物发酵技术（原料药）

Product名称

ACARBOSE阿卡波糖

Guaranteed Values指标

Final titre:

Fermentation time:

8-9g/lt

120-144hrs

Recovery yield: 55%

ACLARUBICIN 阿柔比星

ACRYLAMIDE 丙烯酰胺

Two step fermentation

Final titre:

Fermentation time:

Recovery yield:

AMOXICILLIN 阿莫西林

325 g/lt

40-48 hrs

98%

Yield: ＞90%(guaranteed; semi-continuous process with

recycling)

＞95%(achieved; semi-continuous process with

recycling)

Amphotericin B两性霉素B

Final titre: 10000U/ml(HPLC)

Fermentation time: 130-140hrs

Recovery yield: 70%

ANDROSTENDIONE-SPIRONOLACTO

NE 雄烯二酮-螺内酯

Androstendione Final titre:

Fermentation time:

Recovery yield:

Sprironolactone Synthesis:

20 ± 2 g/lt

96 ± 24 hrs

>90 % as Crystals 96% purity

66% from AD

8 g/l

144 ±12 hrs

≥80%

10 ±1 g/lt

134 hrs

APRAMYCIN Sulfate 硫酸安普霉素

Final titre:

Fermentation time:

Recovery yield:

ARACHIDONIC ACID 花生四烯酸

Final titre:

Fermentation time:

1

Product名称

Guaranteed Values指标

Recovery yield: 80 ± 5%

ARGININE精氨酸

Final titre: 75±5g/L

Fermentation time: 50 hrs

Recovery yield: >80％ as crystal

>90％ feed grade

ASTAXANTHIN 虾青素

Final titre:

Fermentation time:

3 g/lt

180±10 hrs

Recovery yield: 60%

AVERMECTIN阿维菌素

Final titre: 5000 u/ml (+ 10%)

Fermentation time: 260-280 hrs

Recovery yield: > 75%

BACITRACIN-Zinc 杆菌肽锌

Final titre

Fermentation time:

Recovery yield:

BLEOMYCIN 博莱霉素

1200u/mL

36 hrs

≥55 %

Final titre: 140±20mg/L

Fermentation Time: 144±12hrs

Recovery yield: ≥35%

CABERGOLINE卡麦角林

By chemical synthesis from Lysergic acid

3.29kg Cabergoline from 1kg Lysergic acid

-CAROTENE -胡萝卜素

Final titre:

Fermentation time:

Recovery yield as feed quality:

3g/l

96±12 hrs

90 %

28 g/lt

> 30 g/lt

144±24 hrs

>80%

33 g/L

100 hrs

CEPHALOSPORIN C, Na SALT

头孢菌素C钠盐

Final titre:

Pilot plant titer:

Fermentation time:

Recovery yield:

CHLORTETRACYCLINE金霉素

Final titre:

Fermentation time:

Recovery yield: 94 % feed grade, 75 % hydrochloride

2

Product名称

Citric Acid柠檬酸

Guaranteed Values指标

The yield，conversion rate and fermentation time are

different between using different kinds of raw materials.

CLAVULANIC ACID 克拉维酸

COMPACTIN美伐他汀

CYCLOSPORIN A 环孢霉素A

Final titre:

Fermentation time:

Recovery Yield:

Final titre:

Final titre:

Fermentation time:

Recovery yield:

11±1g/lt

140 ± 12 hrs

60±5 %

14 g/l

5 ± 1 g/lt

168 hrs

70 %

1 ± 0.2 g/lt

216±12 hrs

≥50 %

3g±0.3g/lt

168±12 hrs

≥67 %

2.5±0.3 g/lt

196±24hrs

≥40%

15 ±1.5 g/lt

192±12 hrs

35 - 40 %

28 ±3 g/lt

120±24 hrs

DALBAVANCIN PRECURSOR

达巴万星（道古霉素）前体

Final titre:

Fermentation time:

Recovery yield:

DAUNOMYCIN 柔红霉素

Final titre:

Fermentation time:

Recovery yield:

DAPTOMYCIN/CUBICIN

达托霉素

Final titre:

Fermentation time:

Recovery yield Pharma grade:

Final titre:

Fermentation time:

Recovery yield:

Final titre:

Fermentation time:

DESFEROXAMIN

去铁胺

DHA

Recovery yield: >80 %

DMCT/Demethylchlortetracycline

去甲基氯四环素/去甲金霉素

Final titre: 10.0 ± 1g/L

Fermentation time: 300 hrs

Recovery Yield: 75％

DORAMECTIN 多拉霉素

Final titre: 1500UI/ml

Fermentation time: 200 hrs

3

Product名称

Guaranteed Values指标

Recovery Yield: 75％

DOXORUBICIN阿霉素

Antitumor drug

Chemical synth. from Dauno: Yield >50%

ENRAMYCIN 恩拉霉素

Final Titre: 10g/lt

Fermentation Time: 260hrs

Recovery yield: ≥92%

ERYTHROMYCIN 红霉素

Final titre (HPLC): 10 ± 0.5g/lt

Fermentation time: 168+24 hrs

Recovery yield: 85% as Thiocyante. 82% as Base

EPIRUBICIN表阿霉素

Antitumor drug

Chemical synth. from Dauno: Yield >30%

EPOTHILONE B 埃博霉素B

Final titre: 70-90 mg/L

Fermentation time: 168-216 hrs

FLAVOMYCIN黄霉素

Final titre:

Fermentation time:

Recovery yield as feed quality:

14 ±1 g/lt

148 - 172 hrs

>92 %

Fusidic Acid 福西地酸

Final Titre: 3.8g/L

Fermentation Time: 190hrs

Recovery yield: ≥60%

FRUCTO-OLIGO SACCARIDE (FOS)

果寡糖

GENTAMYCIN 庆大霉素

Glutathione(GSH) 谷胱苷肽

GUANOSINE 鸟苷

Contact us for more details

Final titre:

Fermentation time:

Recovery yield:

Final titre:

Fermentation time:

6g/l

14days

80%

5.5±0.5 g/l

65±5 hrs

Recovery yield: 45-50%

Final titre:

Fermentation time:

Sugar conversion rate:

4

50-60 g/l

72 hrs

25%

Product名称

Guaranteed Values指标

Recovery yield: 80%

GIBBERELLIN (GA) 赤霉素

Fermentation loading coefficient：90—95%

Fermentation titre： mix constituents：2200-2400μg/ml；

GA3 constituents：1900—2100μg/ml

Extraction yield：85%

HYALURONIC ACID透明质酸

Final titre:

Fermentation time:

Recovery yield:

6.5 g/litre

20-24 hrs

90 % Cosmetic Grade

≥90 g/l

85-90 hrs

35%

INOSINE 肌苷

Final titre:

Fermentation time:

Sugar conversion rate:

Recovery yield: 80%

KANAMYCIN 卡那霉素

KASUGAMYCIN 春雷霉素

Final titre:

Fermentation time:

30g/l

300 hrs

Recovery yield: 80%

Final titre: 10000-12000U/ml

Fermentation time: 8 days

Recovery yield: > 80%

Final titre:

Fermentation time:

Recovery yield:

120 ±10g/lt

72 ± 10 hrs

>90%

8 g/lt

192 ± 24 hrs

LACTIC ACID 乳酸

LINCOMYCIN 林肯霉素

Final titre:

Fermentation time:

Recovery yield： Pharma grade 80% Feed Grade 90%

LIPSTATIN 泥泊司他汀

Chemical conversion from Lip to

Orlistat

ORLISTAT 奥利司他

LOVASTATIN 洛伐他汀

Final titre:

Fermentation time:

Recovery yield:

Contact us for more details.

Final titre

Fermentation time

Recovery yield

Lycopene番茄红素

17g/lt

264 ± 24 hrs

70 ± 5 %

8 ± 1 g/lt

168 hrs

75%

Titre： 2.5 ± 0.5 g/l

Fermentation time: 120±12 hrs

5

Product名称

LYSERGIC ACID 麦角酸

Guaranteed Values指标

Extraction yield: 75±5%

Final titre

Fermentation time:

Recovery yield:

6 g/l

360 hrs

≥ 70 %

190g/l

45 hrs

LYSINE 赖氨酸

Final titre:

Fermentation time:

Recovery yield: 95 % for crystal (assay >98.5%)

100% for feed grade (assay 65%)

Sugar conversion rate: 55% - 60 %

MADURAMYCIN 马杜拉霉素

Final titre:

Fermentation time

Recovery yield:

MIBEMYCIN 美贝霉素

10’000 – 12’000 UI/ml

180 – 210 hrs.

> 90 % Feed Grade

Final titre: ≥400 U/ml

Extraction yield: 60%-70%

Synthetic yield: 30%-50%

MITHRAYCIN光辉霉素

MONENSIN 莫能菌素

Production Titre： > 500 mg/l

Final titre:

Fermentation time:

32000-40000 u/ml

320 hrs.

Recovery yield: 95%

MUPIROCIN 莫匹罗星

Final Titre： 3000mg/L（50L fermentor）

Fermentation Time： 60hrs

MYCOPHENOLIC ACID 麦考酚酸

Final titre:

Fermentation time:

Recovery yield:

Final titre:

Fermentation time:

NICERGOLINE 尼麦角林

16±1 g/l

312±24hrs

> 68 %

8g/l(Maximum 10g/l)

140-160 hrs.

NATAMYCIN 纳他霉素

Recovery yield: 55-65%

By chemical synthesis from Lysergic acid

2.47kg nicergoline from 1kg Lyserig acid

NYSTATIN制霉菌素

Final titre:

Fermentation time:

＞400,000 IU/ml

324±24 hrs.

Recovery yield: 85 % Feed Grade, 70 % Pharma Grade

6

Product名称

OXYTETRACYCLINE土霉素

Guaranteed Values指标

Final titre:

Fermentation time:

Recovery yield feed grade:

33mg/l

240hrs

94%

6g/lt

156 hrs

70 % as Crystals

80,000 IU/ml

192 hrs

 85 %

PARAMOMYCIN巴龙霉素

Final titre:

Fermentation time:

Recovery yield:

PENICILLIN G POTASSIUM SALT

青霉素G钾盐

Phenylalanine 苯丙氨酸

Final titre:

Fermentation time:

Recovery yield:

Final titre: 32±2g／l

Fermentation time: 45-55 hrs

Recovery yield: >75％

Conversion rate: 14-16％

PLEUROMUTILIN截短侧耳素

Pneumocandin Bo 肺囊康定

New

Chemical conversion from Bo to

caspofungin

CASPOFUNGIN 卡泊芬净

MICAFUNGIN 米卡芬净

ε-Polylysine 聚赖氨酸

Final titre:

Final titre:

Fermentation time:

13 g/lt（50L fermenter）

2.3-3.0g/L

312 hrs

Recovery yield: 70 %

Contact us for more details.

Contact us for more details

Yield of acid by fermentation: 20g/l

Fermentation time： 70hrs

Recovery yield： 80％

POLYMYXIN 粘杆菌素B

Final titre:

Fermentation time:

Recovery yield:

2.5 ±0.2 g/l

168±24 hrs

>70 %

450.000 U/l (Maximum 560,000U/l)

96 hrs±10

POLYMYXIN E Sulfate硫酸粘杆菌素E

Final titre:

Fermentation time

Recovery yield: 93-95% concentrate 80-85% A.P.I.

PRAVASTATIN 普伐他汀

Titre: 15 g/l

Fermentation time: 9-10days

7

Product名称

Guaranteed Values指标

Recovery yield: >65 %

Conversion from Mevastatin : > 70%

Prednisolonum/ Prednisolone

氢化泼尼松/泼尼松龙

Concentration of substrate: >20 g/L (Maximum 70g/L)

Bioconversion time: 5-8 hrs

Substrate conversion rate: 97～100％

Yield of crystalline final product: >80％

PRISTINAMYCINS

普那霉素/原始霉素

COENZYME Q10 辅酶Q10

Final titre:

Fermentation time:

2.5- 3.0g/L

64 hrs.

Recovery yield:

75%

Final titre:

Fermentation time:

Recovery yield:

Final titre:

Recovery yield:

3.5±0.5g/l

120±12 hrs

>50 %

2±0.2 g/L

216±12 hrs

≥50%

> 26 g/lt

192 hrs

Ramoplanin雷莫拉宁

Fermentation time:

RIFAMYCIN-RIFAMPICIN

利福霉素-利福平

Rh- LACTOFERRIN

乳铁蛋白

Final titre:

Fermentation time:

Recovery (up to Rifampycin): >65%

Final titre: 3.5±0.3g/L

Fermentation time: 168±12hrs

Recovery yield: 60±5%

SAMe 腺苷蛋氨酸

Final titre: ≥10g/L

Fermentation time: 16 hrs

SALINOMYCIN 盐霉素

Final titre:

Fermentation Time:

Recovery as Feed Grade:

10 g/lt

360 hrs  24

> 92 %

30 ± 3 g/lt

60 ±12 hrs

SHIKIMIC ACID

莽草酸

SIMVASTATIN 辛伐他汀

Final titre:

Fermentation time:

Recovery yield: 75%

Chemical conversion of Lovastatin

11 – 12 kg Simvastatin from 20 kg Lovastatin

8

Product名称

SIROLIMUS 西罗莫司

Guaranteed Values指标

Final titre:

Fermentation time:

1.2 ± 0.1 g/lt

216 ±24 hrs

Recovery yield: >50%

SPECTINOMYCIN 大观霉素

Final titre:

Recovery yield:

SPIRAMYCIN/Rovamycin 螺旋霉素

Final titre:

Fermentation Time:

4000u/ml

65-70 %

5000r/m

144 hrs

Recovery yield: 76%

STREPTOMYCIN 链霉素

Final titre:

Fermentation time:

Recovery yield:

TACROLIMUS 他克莫司

TEICOPLANIN替考拉宁

Testosterone睾丸激素

Final titre:

Fermentation time:

Recovery yield:

Final titre:

Fermentation time:

Recovery:

24 g/l

200 hrs

72 %

1.2 g/l

240 + 24hrs

> 70 %

5+ 0.5 g/l

168 + 12 hrs

 55 %

Concentration of substrate: 5.0 g/L

Bioconversion time: 135～145 hrs

content in cultivation broth: 48～50％

Yield of crystalline final product: 30～35％

TETRACYCLINE HCI 盐酸四环素

Final titre:

Fermentation time:

Recovery yield:

>30 g/l

200 hrs

74 %

85-95 g/l

35-40 hrs

THREONINE苏氨酸

Final titre:

Fermentation time:

Recovery: 80%

THIAMULIN Base泰妙菌素碱

Conversion rate:

From 1 kg Pleuromutilin to 1,23 kg Thiamulin Fumarate

TOBRAMYCIN 妥布霉素

Final titre:

Fermentation time:

6000r/ml

120-144 hrs

Recovery yield: 30 %

9

Product名称

TRYPTOPHAN 色氨酸

Guaranteed Values指标

Final titre:

Fermentation time:

35±3 g/L

50±5 hrs

≥70%

≥85%

Recovery yield as crystal:

Recovery yield as Feed Grade:

Glucose conversion rate: 12-15％

TYLOSIN TARTRATE 酒石酸泰乐菌素

Final titre:

Fermentation time:

Recovery yield:

15± 1 g/lt.

180±12 hrs

≥70%

60±5 g/l

60 ± 5 hrs

VALINE 缬氨酸

Final titre:

Fermentation time:

Recovery yield Pharma grade: ≥65%

VANCOMYCIN 万古霉素

Final titre:

Fermentation time:

Recovery yield:

VITAMINE B

12

维生素V B

12

VITAMINE B

2

Final titre:

Fermentation time:

Recovery yield:

Final titre:

Fermentation time:

80 % as Feed Grade

XANTHAN 黄原胶

AMPICILLIN氨苄青霉素 (CAS

69-53-4)

CEPHALEXIN头孢氨苄(CAS

15686-71-2)

CEPHADROXIL 头孢羟氨苄(CAS

50370-12-2)

Bio-Enzymatic Reaction

Bio-Enzymatic Reaction

Final titre:

Recovery yield:

Fermentation Time

Bio-Enzymatic Reaction

25 kg/m3

80 %

36 hrs

12 ± 1 g/l

180 ± 12hrs

70 %

220 g/L

168 hrs

> 70% as Crystal, 80% as Feed Grade

33 ± 3g/l

48 ± 24 hrs

Recovery yield: 65 % as Pharma Grade,

10

Product名称

D-p-OH-Phenylglycine-amide

D-p-OH-Phenylglycine-ester

L-tert-leucine L-亮叔氨酸

L-homophenylalanine L-高苯丙氨酸

Guaranteed Values指标

Bio-Enzymatic Reaction

Bio-Enzymatic Reaction

Biocatalytic method

Biocatalytic method

TECHNOLOGIES OF ENZYMES PRODUCTION

（HIGH） ALKALINE PROTEASE

碱性蛋白酶

Cellulase 纤维素酶

Final titre:

Fermentation time:

Activity of enzyme in harvest

broth when release: 60-80 IU/ml

Fermentation time:

Recovery yield:

Dextranse 葡聚糖酶

140-150 hrs

85－90％

30000 U/ml

50 hrs

Fermentation titre： 10000 U/ml

flask 1000u/ml

Heparanase

肝素酶

Heparanase activity:

Fermentation time:

20000IU/L

24±5 hrs

Recovery yield: 65%

Lipase脂肪酶

PENICILLIN ACYLASE 青霉素酰化酶

Fermentation titre： 1500－2000 FIPU/ml

Fermentation Time： 72－96 hrs

Recovery yield： 70－90

Enzyme activity: 250-260 u/g (28°C dry basis)

Final titre: 50-60u/ml (28°C)

Fermentation time: 120-140 hrs

Recovery yield: ≥40%

THERMOSTABILITY AMYLASE

Fermentation time: 150hrs

Activity: 100,000,000 U/mL

11

耐高温淀粉酶

GLUCOAMYLASE 葡糖淀粉酶

Fermentation time: 180hrs

Activity: 100,000 U/mL

Xylanase木聚糖酶

Incision enzyme activity: 120U/ml

Excision enzymeactivity: 60－80U/ml

Fermentation time: 72 hrs

Recovery yield:

PHYTASE 植酸酶

70－90%

Enzyme activity: 1000 ~ 5000u/g (37 ℃, pH 5.5)

Final titre: 20000u/ml

Fermentation time: 200-250hrs

Recovery yield: 70-80%

CHEMICAL SYNTHETIC/SEMI-SYNTHETIC PRODUCTION

XYLITOL 木糖醇

Fermentation from xylose mother

liquid to L-Arabinose

L-ARABINOSE L-阿拉伯糖

Purity: ≥93%

Contact us for more details

12